This contains 100% correct material for UMUC Biology 103 LAB01. However, this is an Answer Key, which means, you should put it in your own words. Here is a sample for the questions answered:

Exercise 1: Data Interpretation  (2 pts each)

1. What patterns do you observe based on the information in Table 4?

No fish are present when the dissolved oxygen is zero. When there is more dissolved oxygen in the water, more fish are present. However, the number of fish tends to drop or level off when the dissolved oxygen is higher than 12 ppm.

 

2. Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water.

Possible Hypotheses:

1.    The amount of dissolved oxygen affects the number of fish that can live in a body of water.

2.    As dissolved oxygen concentration increases, more fish can live in the body of water.

3.    There is an ideal dissolved oxygen concentration for fish to live in.

 

The rest of the questions are answered in full version:

1.    What would your experimental approach be to test this hypothesis?

 

 

2.    What would be the independent and dependent variables?

 

 

3.    What would be your control?

 

 

4.    What type of graph would be appropriate for this data set?  Why?

 

 

5.    Graph the data from Table 4: Water Quality vs. Fish Population (found at the beginning of this exercise).

 

 

6.    Interpret the data from the graph made in Question 7.

 

 

 

Exercise 2: Experimental Variables

Determine the variables tested in the each of the following experiments. If applicable, determine and identify any positive or negative controls.

 

Observations

1.    A study is being done to test the effects of habitat space on the size of fish populations. Different sized aquariums are set up with six goldfish in each one. Over a period of six months, the fish are fed the same type and amount of food. The aquariums are equally maintained and cleaned throughout the experiment. The temperature of the water is kept constant. At the end of the experiment the number of surviving fish is surveyed.

A.    Independent Variable:

 

 

B.    Dependent Variable:

 

 

C.   Controlled Variables/Constants:

 

 

D.   Experimental Controls/Control Groups:

 

 

2.    To determine if the type of agar affects bacterial growth, a scientist cultures E. coli on four different types of agar. Five petri dishes are set up to collect results:

§  One with nutrient agar and E. coli

§  One with mannitol-salt agar and E. coli

 

§  One with MacConkey agar and E. coli

§  One with LB agar and E. coli

§  One with nutrient agar but NO E. coli

 

All of the petri dishes received the same volume of agar, and were the same shape and size. During the experiment, the temperature at which the petri dishes were stored, and at the air quality remained the same. After one week the amount of bacterial growth was measured.

A.    Independent Variable:

 

 

B.    Dependent Variable:

 

C.   Controlled Variables/Constants:

 

 

D. Experimental Controls/Control Groups:

Exercise 3: Testable Observations

Determine which of the following observations are testable. For those that are testable:

Determine if the observation is qualitative or quantitative

Write a hypothesis and null hypothesis

What would be your experimental approach?

What are the dependent and independent variables?

What are your controls – both positive and negative?

How will you collect your data?

How will you present your data (charts, graphs, types)?

How will you analyze your data?

 

Observations

1.    A plant grows three inches faster per day when placed on a window sill than it does when placed on a on a coffee table in the middle of the living room.

 

 

2.    The teller at the bank with brown hair and brown eyes is taller than the other tellers.

 

 

 

3.    When Sally eats healthy foods and exercises regularly, her blood pressure is 10 points lower than when she does not exercise and eats fatty foods.

 

 

 

4.    The Italian restaurant across the street closes at 9 pm but the one two blocks away closes at 10 pm.

 

 

5.    For the past two days, the clouds have come out at 3 pm and it has started raining at 3:15 pm.

 

 

 

6.    George did not sleep at all the night following the start of daylight savings.

 

 

 

Exercise 4: Conversion

For each of the following, convert each value into the designated units.

 

 

1.    46,756,790 mg = _______ kg

 

 

2.    5.6 hours = ________ seconds

 

 

3.    13.5 cm = ________ inches

 

 

4.    47 °C = _______ °F

 

 

 

 

Exercise 5: Accuracy vs. Precision

For the following, determine whether the information is accurate, precise, both or neither.

 

1.    During gym class, four students decided to see if they could beat the norm of 45 sit-ups in a minute. The first student did 64 sit-ups, the second did 69, the third did 65, and the fourth did 67.

 

 

2.    The average score for the 5th grade math test is 89.5. The top 5th graders took the test and scored 89, 93, 91 and 87.

 

3.    Yesterday the temperature was 89 °F, tomorrow it’s supposed to be 88 °F and the next day it’s supposed to be 90 °F, even though the average for September is only 75 °F degrees!

 

4.    Four friends decided to go out and play horseshoes. They took a picture of their results shown to the right:

 

 

 

 

5.    A local grocery store was holding a contest to see who could most closely guess the number of pennies that they had inside a large jar. The first six people guessed the numbers 735, 209, 390, 300, 1005 and 689. The grocery clerk said the jar actually contains 568 pennies.

 

 

Exercise 6: Significant Digits and Scientific Notation

Part 1: Determine the number of significant digits in each number and write out the specific significant digits.

 

1.    405000

 

 

2.    0.0098

 

 

3.    39.999999

 

 

4.    13.00

 

 

5.    80,000,089

 

 

6.    55,430.00

 

 

7.    0.000033

 

 

8.    620.03080

 

Part 2: Write the numbers below in scientific notation, incorporating what you know about significant digits.

 

1.    70,000,000,000

 

 

2.    0.000000048

 

 

3.    67,890,000

 

 

4.    70,500

 

 

5.    450,900,800

 

 

6.    0.009045

 

 

7.    0.023

NEED HELP WITH BLANK BOX QUESTION

(question 1 and 4 in conclusion part. )

* It would be nice if you can check my answeres too.

NOTES:

Tube Sample Lane

1 1 kb DNA ladder 1

2 Mother’s DNA 2

3 Child’s DNA 3

4 A.F. #1 DNA 4

5 A.F. #2 DNA 5

 

 

 

Record the distance each ladder band moved from the well in mm along with the size of the DNA fragments in that band in bp units, based on the bp given in step 19 , in your Lab Notes.

 

Lane 1:

 

1,000 bp 34 mm

900 bp 36 mm

800 bp 38 mm

700 bp 40 mm

600 bp 43 mm

500 bp 47 mm

400 bp 51 mm

300 bp 56 mm

250 bp 60 mm

200 bp 64 mm

150 bp 69 mm

100 bp 77 mm

50 bp 90 mm

 

 

Lane 2:

 

37 mm 850bp

59 mm 265bp

 

 

Lane 3:

 

37 mm 850bp

44 mm 575bp

 

Lane 4:

 

41 mm 670bp

43 mm 600bp

 

Lane 5:

 

44 mm 575bp

 

55 mm 320bp

 

Experiment: Agarose Gel Electrophoresis of DNA Fragments

Lab Results

1. List the distances traveled in mm for the bands in the DNA ladder in the table below.
   Remember, smaller fragments travel farther than longer ones, so the top-most band will be the 1,000 bp sized DNA fragments whereas the bottom-most band will be the 50 bp sized DNA fragments. 

   DNA Ladder
   Band	Distance (mm)
   50 bp	90 mm
   100 bp	77 mm
   150 bp	69 mm
   200 bp	64 mm
   250 bp	60 mm
   300 bp	56 mm
   400 bp	51 mm
   500 bp	47 mm
   600 bp	43 mm
   700 bp	40 mm
   800 bp	38 mm
   900 bp	36 mm
   1,000 bp	34 mm

    

2. Whose sample had the approximately 570 bp and 320 bp sized DNA fragments?
   

   A.F. #2

3. What were the sizes of the DNA fragments for alleged father #1?
   

   41 mm 670bp 43 mm 600bp

Data Analysis

1. Which size DNA fragment did the child inherit from her mother?
   

   37 mm 850bp

2. Which alleged father, if any, can be definitively ruled out as the child’s biological father?
   

   A.F. #2

Conclusions

1. How are new molecules of DNA synthesized in living cells?
   
2. What is the function of DNA?
   

   DNA has genetic information that controls our cells. So, DNA is like a blueprint that shows how to construct components of cells like proteins and ribonucleic acid (RNA). This information is carried down to newer generations through inheritance.

3. If each individual has such a small amount of DNA in their cells, how do the bands on the gel contain enough DNA to be visible?
   

   In order to make DNA visible. The Gel has to be soaked in a dye (ethidium bromide) to bind with the DNA and rinsed off after. Ethidium bromide helps to make DNA visible by glowing brightly in UV rays.

4. Humans only have a few eye colors and only four ABO-based blood types. How can DNA tests definitively identify individuals when many people have brown eyes or type A blood?
   
5. Suppose a suspicious hair was found in a victim’s home. A gel is set up with the DNA fragments of two suspected criminals in lanes 4 and 5, the DNA fragments of the suspicious hair in lane 3, and the victim’s DNA fragments, as a negative control, are in lane 2. A DNA ladder is in lane 1. The resulting gel is below. Which suspect, if any, committed the crime? Explain your answer.
   

   Lane 4,5 (two suspected criniminals)