Describe the individual importance of each statement to the organization

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Assignment Description:Your chief executive officer (CEO) has just notified you that the board wants the health care organization to update the mission, vision, and values of the organization. The CEO asks that you complete the following in 2-4 pages not including title page and reference page.• Create a mission, vision, and values statement for the healthcare organization.• Provide the definition and meaning of each statement.• Describe the individual importance of each statement to the organization.• Discuss methods of communicating those statements to all staff members.• Discuss organizational leaders can evaluate each statement’s effectiveness.

Concentration Gradients and Membrane Permeability

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Experiment 2: Concentration Gradients and Membrane Permeability

In this experiment, you will dialyze a solution of glucose and starch to observe:

  • The directional movement of glucose and starch.
  • The effect of a selectively permeable membrane on the diffusion of these molecules.

An indicator is a substance that changes color when in the presence of a specific substance. In this experiment, IKI will be used as an indicator to test for the presence of starch.

 

Materials

(5) 100 mL Beakers
10 mL 1% Glucose Solution, C6H12O6
4 Glucose Test Strips
(1) 100 mL Graduated Cylinder
4 mL 1% Iodine-Potassium Iodide, IKI
5 mL Liquid Starch, C6H10O5
3 Pipettes
4 Rubber Bands (Small; contain latex, handle with gloves on if allergic)

  

 

Permanent Marker
*Stopwatch
*Water
*Scissors

*15.0 cm Dialysis Tubing

*You Must Provide
*Be sure to measure and cut only the length you need for this experiment. Reserve the remainder for later experiments.

 

 

 

Attention!

Do not allow the open end of the dialysis tubing to fall into the beaker. If it does, remove the tube and rinse thoroughly with water before refilling it with the starch/glucose solution and replacing it in the beaker.

 
Note:

If you make a mistake, the dialysis tubing can be rinsed and used again.

Dialysis tubing must be soaked in water before you will be able to open it up to create the dialysis “bag.” Follow these directions for this experiment:

1.      Soak the tubing in a beaker of water for ten minutes.

2.     Place the dialysis tubing between your thumb and forefinger, and rub the two digits together in a shearing manner. This motion should open up the “tube” so that you can fill it with the different solutions.

 

Procedure

1.     Measure and pour 50 mL of water into a 100 mL beaker using the 100 mL graduated cylinder. Cut a piece of dialysis tubing 15.0 cm long. Submerge the dialysis tubing in the water for at least ten minutes.

2.     Measure and pour 82 mL of water into a second 100 mL beaker using the 100 mL graduated cylinder. This is the beaker you will put the filled dialysis bag into in Step 9.

3.     Make the glucose/sucrose mixture. Use a graduated pipette to add 5 mL of glucose solution to a third 100 mL beaker and label it “dialysis bag solution.” Use a different graduated pipette to add 5 mL of starch solution to the same beaker. Mix by pipetting the solution up and down six times.

4.     Using the same pipette that you used to mix the dialysis bag solution, remove 2 mL of the dialysis bag solution and place it in a clean beaker. This sample will serve as your positive control for glucose and starch.

a.     Dip one of the glucose test strips into the 2 mL of glucose/starch solution in the third beaker. After one minute has passed, record the final color of the glucose test strip in Table 3. This is your positive control for glucose.

b.     Use a pipette to transfer approximately 0.5 mL of IKI into the 2 mL of glucose/starch solution into the third beaker. After one minute has passed, record the final color of the glucose/starch solution in the beaker in Table 3. This is your positive control for starch.

5.     Using a clean pipette, remove 2 mL of water from the 82 mL of water you placed in a beaker in Step 2, and place it in a clean beaker. This sample will serve as your negative controls for glucose and starch.

a.     Dip one of the glucose test strips into the 2 mL of water in the beaker. After one minute has passed, record the final color of the glucose test strip in Table 3. This is your negative control for glucose.

b.     Use a pipette to transfer approximately 0.5 mL of IKI into the 2 mL in the beaker. After one minute has passed, record the final color of the water in the beaker      in Table 3. This is your negative control for starch.

Note:The color results of these controls determine the indicator reagent key. You must use these results to interpret the rest of your results.

6.     After at least ten minutes have passed, remove the dialysis tube and close one end by folding over 3.0 cm of one end (bottom). Fold it again and secure with a rubber band (use two rubber bands if necessary).

7.     Test to make sure the closed end of the dialysis tube will not allow solution to leak out. Dry off the outside of the dialysis tube bag with a cloth or paper towel. Then, add a small amount of water to the bag and examine the rubber band seal for leakage. Be sure to remove the water from the inside of the bag before continuing.

  1. Using the same pipette that was used to mix the solution in Step 3, transfer 8 mL of the dialysis bag solution to the prepared dialysis bag.
Figure 4: Step 9 reference.
Figure 4:Step 9 reference.

9.     Place the filled dialysis bag in the 100 mL beaker filled with 80 mL of water, leaving the open end draped over the edge of the beaker as shown in Figure 4.

10.Allow the solution to sit for 60 minutes. Clean and dry all materials except the beaker holding the dialysis bag.

11.After the solution has diffused for 60 minutes, remove the dialysis bag from the beaker and empty the contents of the bag into a clean, dry beaker. Label the beaker “final dialysis bag solution.”

12.Test the final dialysis bag solution for the presence of glucose by dipping one glucose test strip into the dialysis bag. Wait one minute before reading the results of the test strip. Record your results for the presence of glucose in Table 4.

13.Test for the presence of starch by adding 2 mL IKI. After one minute has passed, record the final color in Table 4.

14.Use a pipette to transfer 8 mL of the water in the beaker to a clean beaker. Test the beaker water for the presence of glucose by dipping one glucose test strip into the beaker. Wait one minute before reading the results of the test strip, and record the results in Table 4.

15.Test for the presence of starch by adding 2 mL of IKI to the beaker water. Record the final color of the beaker solution in Table 4.

 

 

 

Table 3: Indicator Reagent Data
Indicator Starch Positive
Control (Color)		 Starch Negative
Control (Color)		 Glucose Positive
Control (Color)		 Glucose Negative
Control (Color)
Glucose Test Strip n/a n/a
IKI Solution n/a n/a

 

Table 4: Diffusion of Starch and Glucose Over Time
Indicator Dialysis Bag After 60 Minutes Beaker Water After 60 Minutes
IKI Solution
Glucose Test Strip

 

Post-Lab Questions

1.     Why is it necessary to have positive and negative controls in this experiment?

 

 

 

 

 

2.     Draw a diagram of the experimental set-up. Use arrows to depict the movement of each substance in the dialysis bag and the beaker.

 

 

 

 

3.     Which substance(s) crossed the dialysis membrane? Support your response with data-based evidence.

 

 

 

 

4.     Which molecules remained inside of the dialysis bag?

 

 

5.     Did all of the molecules diffuse out of the bag into the beaker? Why or why not?

 

 

 

 

 

Experiment 1: Diffusion through a Liquid

In this experiment, you will observe the effect that different molecular weights have on the ability of dye to travel through a viscous medium.

Materials

1 60 mL Corn Syrup Bottle, C12H22O11
Red and Blue Dye Solutions (Blue molecular weight = 793 g/mole; red molecular weight = 496 g/mole)
(1) 9 cm Petri Dish (top and bottom halves)

  

Ruler
*Stopwatch
*Clear Tape

*You Must Provide

 

Procedure

1.     Use clear tape to secure one-half of the petri dish (either the bottom or the top half) over a ruler. Make sure that you can read the measurement markings on the ruler through the petri dish. The dish should be positioned with the open end of the dish facing upwards.

  1. Carefully fill the half of the petri dish with corn syrup until the entire surface is covered.
  2. Develop a hypothesis regarding which color dye you believe will diffuse faster across the corn syrup and why. Record this in the post-lab questions.
  3. Place a single drop of blue dye in the middle of the corn syrup. Note the position where the dye fell by reading the location of its outside edge on the ruler.
  4. Record the location of the outside edge of the dye (the distance it has traveled) every ten seconds for a total of two minutes. Record your data in Table 1 and use your results to perform the calculations in Table 2.
  5. Repeat the procedure using the red dye, the unused half of the petri dish, and fresh corn syrup.

 

Table 1: Rate of Diffusion in Corn Syrup
Time (sec) Blue Dye Red Dye Time (sec) Blue Dye Red Dye
10 70
20 80
30 90
40 100
50 110
60 120

 

Table 2: Speed of Diffusion of Different Molecular Weight Dyes
Structure Molecular Weight Total Distance
Traveled (mm)		 Speed of Diffusion
(mm/hr)*
Blue Dye
Red Dye

*Multiply the total distance diffused by 30 to get the hourly diffusion rate

 

Post-Lab Questions

  1. Record your hypothesis from Step 3 here. Be sure to validate your predictions with scientific reasoning.

 

 

 

  1. Which dye diffused the fastest?

 

 

 

  1. Does the rate of diffusion correspond with the molecular weight of the dye?

 

 

 

 

 

  1. Does the rate of diffusion change over time? Why or why not?

 

 

 

 

  1. Examine the graph below. Does it match the data you recorded in Table 2? Explain why, or why not. Submit your own plot if necessary.

 

https://nuonline.neu.edu/bbcswebdav/pid-9451339-dt-content-rid-14232100_1/courses/BIO1101.90155.201714/BIO1101.90155.201714_ImportedContent_20160930044714/CourseRoot/html/lab006s001.html

 

 

https://nuonline.neu.edu/bbcswebdav/pid-9451340-dt-content-rid-14232401_1/courses/BIO1101.90155.201714/BIO1101.90155.201714_ImportedContent_20160930044714/CourseRoot/html/lab006s002.html

 

https://nuonline.neu.edu/bbcswebdav/pid-9451341-dt-content-rid-14232402_1/courses/BIO1101.90155.201714/BIO1101.90155.201714_ImportedContent_20160930044714/CourseRoot/html/lab006s003.html

 

Prepare a management report of approximately 2000 words analysing organisational or the industry-sector identified

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Prepare a management report of approximately 2000 words analysing organisational or the industry-sector identified in Part One – your syndicate presentation. The word count excludes the executive summary, bibliography and other appendices. This report should assess the impact of macro-environmental and competitive external factors on the organisation/industry-sector and evaluate the potential organizational competitive responses to the primary issues affecting it. Task Outline the primary external influences to which the organisation/industry-sector is subject. Justify your identification of these drivers and include a short discussion of three scenarios that link directly to your environmental analysis. This report should evaluate the organisation’s potential competitive responses to the primary issues affecting it and draw on (not repeat) the PESTEL and Five Forces analysis from presentation.(see attachment 2 ) . The use of tables and figures to save word count is encouraged. The use of a framework such as McKinsey’s 7S . This will require an extensive analysis of the firm’s strengths and weaknesses and the construction of a SWOT table.

Why do cells which lack cell cycle control exhibit karyotypes which look physically different than cells with normal cell cycle.

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This contains 100% correct material for UMUC Biology 103 LAB05. However, this is an Answer Key, which means, you should put it in your own words. Here is a sample for the Pre lab questions answered:

Pre-Lab Questions

 

 

 

1. What major events occur during interphase?

 

The cell functions at its job, and prepares for mitosis by collecting resources and duplicating organelles (G1) and genetic content (S), then creating proteins needed for nuclear division (G2).

 

 

 

2. A person, residing in a location where they are exposed to the sun often, develops a mutation in some of their skin cells resulting in cancer. Consider whether their offspring will be born with the same mutation. Use scientific evidence to support your answer.

 

 

 

It would be highly unlikely that the person’s offspring will be born with same skin cancer mutation because the mutation occurred in the person’s skin cells. Skin cells are somatic cells (body cells) and are not involved in meiosis or reproduction. For the mutation to be passed on to the offspring, a sex cell (sperm or egg) would have to carry the mutation.

 

The other questions that will be answered:

 

Experiment 1: Following Chromosomal DNA Movement through Meiosis

 

Data Tables and Post-Lab Assessment

 

Trial 1 – Meiotic Division Beads Diagram:

 

Prophase I

 

Metaphase I

 

Anaphase I

 

Telophase I

 

Prophase II

 

Metaphase II

 

Anaphase II

 

Telophase I

 

Cytokinesis

 

Trial 2 – Meiotic Division Beads Diagram:

 

Prophase I

 

Metaphase I

 

Anaphase I

 

Telophase I

 

Prophase II

 

Metaphase II

 

Anaphase II

 

Telophase I

 

Cytokinesis

 

 

 

Post-Lab Questions

 

1.    What is the ploidy of the DNA at the end of meiosis I? What about at the end of meiosis II

2.    How are meiosis I and meiosis II different?

3.    Why do you use non-sister chromatids to demonstrate crossing over?

 

4.    What combinations of alleles could result from a crossover between BD and bd chromosomes?

 

5.    How many chromosomes were present when meiosis I started?

 

6.    How many nuclei are present at the end of meiosis II? How many chromosomes are in each?

 

7.    Identify two ways that meiosis contributes to genetic recombination.

 

8.    Why is it necessary to reduce the number of chromosomes in gametes, but not in other cells?

 

9.    Blue whales have 44 chromosomes in every cell. Determine how many chromosomes you would expect to find in the following:

 

 

 

i.    Sperm Cell:

 

 

ii.    Egg Cell:

 

 

 

iii.    Daughter Cell from Mitosis:

 

 

 

iv.    Daughter Cell from Meiosis II:

 

 

 

10.  Research and find a disease that is caused by chromosomal mutations. When does the mutation occur? What chromosomes are affected? What are the consequences?

 

 

 

 

 

 

 

11.  Diagram what would happen if sexual reproduction took place for four generations using diploid (2n) cells.

 

 

Experiment 2: The Importance of Cell Cycle Control

 

Data Tables and Post-Lab Assessment

 

1.

 

 

 

 

 

2.

 

 

 

 

 

3.

 

 

 

 

 

4.

 

 

 

 

 

5.

 

 

 

 

 

Post-Lab Questions

 

1.    Record your hypothesis from Step 1 in the Procedure section here.

 

 

 

 

 

 

 

2.    What do your results indicate about cell cycle control?

 

 

 

 

 

 

 

3.    Suppose a person developed a mutation in a somatic cell which diminishes the performance of the body’s natural cell cycle control proteins. This mutation resulted in cancer, but was effectively treated with a cocktail of cancer-fighting techniques. Is it possible for this person’s future children to inherit this cancer-causing mutation? Be specific when you explain why or why not.

 

 

 

 

 

 

 

4.    Why do cells which lack cell cycle control exhibit karyotypes which look physically different than cells with normal cell cycle.

 

 

 

 

 

 

 

5.    What are HeLa cells? Why are HeLa cells appropriate for this experiment?