What protein will be the “prey”? That is, if the NES in your newly-discovered protein functions in the typical nuclear export pathway shown in the schematic, what protein will bind to the NES to mediate its export from the nucleus?

/in /by

Topic: Nuclear Transport

NES ОО 5 (a)

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1) What protein will be the “prey”? That is, if the NES in your newly-discovered protein functions in the typical nuclear export pathway shown in the schematic, what protein will bind to the NES to mediate its export from the nucleus?

2) What protein (shown in the schematic) will dissociate the protein-NES complexassuming typical exportin mediated export?

3) Fill out the schematic of the Coomassie-stained SDS-PAGE gel to illustrate the expected results if you used the tag-fragment fusion proteins listed; assume the NES in your newly-discovered protein functions in the typical NES-mediated nuclear export pathway?

) Ran-GTP + Tag- + 1 + - ) Tag-(__ + + + + + + + - + a) + b) + Tag-fragment Fusion protein

NES ОО 5 (a)
) Ran-GTP + Tag- + 1 + – ) Tag-(__ + + + + + + + – + a) + b) + Tag-fragment Fusion protein

 

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