In your immunology laboratory course, your professor has always used her serum as a negative control for the antinuclear antibody test. She had only 0.4 mL remaining, so she aliquoted 0.2 mL for the dilutions to be made for the students sitting at tables 1 and 2 in the lab class. For the students at table 3, her blood was drawn, and a new serum was prepared

Case 2 In your immunology laboratory course, your professor has always used her serum as a negative control for the antinuclear antibody test. She had only 0.4 mL remaining, so she aliquoted 0.2 mL for the dilutions to be made for the students sitting at tables 1 and 2 in the lab class. For the students at table 3, her blood was drawn, and a new serum was prepared. Table 2 also used an aliquot of the new serum to have a second negative control because the students had an extra well-remaining. Although your professor’s samples from last year were negative, this year students at both table 2 and 3 see speckled staining at a 1:64 dilution of the professor’s serum sample. 1. What should be done next?

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