(91572) Assessment 4: Practical Report

Produce a short technical report in a form consistent with proteomic journals covering the techniques, results and interpretation of your proteomics experiment in the practical classes. The report should be in the format of a short communication to a journal; divided into Introduction, Results and Conclusions.

Length: 2000-3000 words.

Weight: 27%

Due: Monday 28 October 2019

Criteria:The report will be assessed on your ability to

• Produce a document of a standard suitable for publication in a designated proteomic journal as a short communication (Professional skills and their appropriate application).
• Include an Introduction that clearly and concisely presents the experimental problem with reference to relevant current literature (Disciplinary knowledge and its appropriate application).
• Methods.
• Include Results that demonstrate the ability to present proteomic data in a discipline appropriate manner (Professional skills and their appropriate application).
• Include a Conclusion that demonstrates the level of expertise gained in Disciplinary knowledge and its appropriate application, Professional skills and their appropriate application and Communication skills, and will evaluate the results in the context of the published literature.

To provide more of a guideline of what you should include in your report.PLEASE NOTE: This next section is a GUIDE. It is NOT a template to be followed to the letter. Use your own judgement as to what you should include and whether you should write MORE to demonstrate your knowledge and understanding.

• In order for the assessors to assess whether or not you have a deep understanding of proteomics in general and of your data, you MUST show the assessors that you understand. Do not assume that your writing will be interpreted by the assessors in a different way to what is written. The assessors can only read what you have written NOT what you intended to say. Be clear and include all of the information necessary to demonstrate your understanding.
• The introduction should provide a small amount of detail about Escherichia coli to establish some context for your report. But the majority of the introduction should describe what is the purpose of analysing a proteome, what are the difficulties encountered when trying to analyse a proteome, how different techniques and technologies can be applied to analyse a proteome, and how quantification of a proteome can be performed. The introduction should end with the aims of the experiments being performed, the main aim being ‘what was done to the treatment sample?’ (but you can word this better).
• Methods. Rather than writing a traditional methods section detailing exactly what you did, your methods section should detail which methods were used and WHY they were used with a focus on how the methods can generate overlapping and complementary data. For example, you performed two separate protein extraction methodologies. WHY was this done? What was the benefits of doing separate extraction methods and why not do just one?
• Results. There are two parts to this section.
  1. You MUST present your own results that you generated. The results of your assays. Your 1D-PAGE images. Your 2D-PAGE images. Your gel images should be labelled to obvious changes in protein abundance and these should be discussed in the text. A summary of your LC/MS/MS data as analysed by Peaks and Mascot, noting any differences. If you were able to performed quantitative analysis, the results of these should be presented. If any other bioinformatic interpretation has been performed (for example, pathway analysis) this should be reported.
  2. If necessary, you can use the data generated by the demonstrators to perform quantitative analysis to produce a list of proteins that are changing in abundance. This list can then be used to answer the questions you set out in your aims. You must list the fold changes in abundance of the proteins that you use as evidence to support your view of what the treatment was.
• Discussion. This can also be broken up into a few sections.
  1. What was the quality of your data like? What steps could you use to improve the data? What additional work, using the current methodologies, could you perform to improve the data quality and reproducibility?
  2. What was done to the treatment sample? What is the evidence for this conclusion? How sure are you of the conclusion? What further experiments would you perform to be more certain?
  3. Were the methods used to generate the data appropriate? Why or why not? Did they produce overplapping, complementary data?
• Conclusion. Wrap everything up concisely. Summarise what you wanted to know, how you went about finding out, whether the analysis is robust and what the data says.
• All of the above should include proper referencing where appropriate. It is not appropriate to say ‘because Matt/Joel/Iain said so’ or ‘it is written in Canvas’. Find appropriate references to support your statements.
• In every section, you need to demonstrate critical thinking and understanding. Show the assessors your knowledge.

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Ashford University Assignment Submision Week 1 Assignment

Initial Argument Paper

This assignment is not an essay but an exercise that helps build toward your Final Paper for the course. Your task in this assignment is to construct the best argument you can for a position on a controversial topic. Your argument should be your own creation. You may take inspiration from other arguments, but the formulation of the argument you present should be original to you.

Prepare:

• Begin by choosing a topic from the PHI103 Final Paper Options list.
• Construct an argument for a position on the topic. This is the position that you will defend in your Final Paper. Make your argument as high quality as possible: In particular, make sure that all of your premises are true and that the truth of the conclusion is demonstrated by your premises.
• Consider possible objections to your argument, and revise it several times until you have an argument that is as strong as possible.
• Search in the Ashford University Library for quality academic sources that support some aspect of your argument.
• Constructing quality arguments is harder than it sounds; you may find that your first few attempts have problems and that it takes quite a bit of time and effort to revise an argument to a point that the premises adequately support the conclusion. You will be revising this argument for each of the remaining assignments in this course.

Write: In your paper,

• Present a main argument in standard form with each premise and the conclusion on a separate line. Here is an example of what it means for an argument to be presented in standard form:
  

  All men are mortal.

  Socrates is a man.

  Therefore, Socrates is mortal.

• Provide support for each premise of your argument. Explain the meaning of the premise, and provide supporting evidence for the premise. [One paragraph for each premise]
• Pay special attention to those premises that could be seen as controversial. Evidence may include academic research sources, supporting arguments, or other ways of demonstrating the truth of the premise (for more ideas about how to support the truth of premises take a look at the instructor guidance for this week). This section should include at least one scholarly research source. For further information about discovering and including high-quality research take a look at the [email protected] Tutorial.
• Explain how your conclusion follows from your premises.
• For an example of how to complete this paper, take a look at the Week One Annotated Example The Ethics of Elephants in Circuses. Let your instructor know if you have any remaining questions about how to complete this paper.

Writing specialists are here 24/7, every day of the year, ready to support you! Click HERE to instantly chat with an online tutor. Click HERE to submit your paper for a review. Papers are returned within 24 hours with a revision plan. Click HERE to email us any writing questions. For additional writing resources like Grammarly, click on the Writing Center tab in the left navigation pane.

For guidance about how to paraphrase sources visit the Ashford Writing Center Guidelines for Paraphrasing Sources webpage.  For guidance about how to quote sources visit the Ashford Writing Center Integrating Quotes Into Your Essay webpage. For guidance about summarizing visit the Ashford Writing Center Guidelines for Summarizing Sources webpage.

The Initial Argument Paper

• Must be 400 to 600 words in length (not including the title and references pages) and formatted according to APA style as outlined in the Ashford Writing Center (for more information about using APA style, take a look at the APA Essay Checklist for Students.
• Must include a separate title page with the following:

• Title of paper
• Studentâ€s name
• Course name and number
• Instructorâ€s name
• Date submitted

• Must use at least one scholarly source in addition to the course text.

• The Scholarly, Peer Reviewed, and Other Credible Sources table offers additional guidance on appropriate source types. If you have questions about whether a specific source is appropriate for this assignment, please contact your instructor. Your instructor has the final say about the appropriateness of a specific source for a particular assignment.

• Must document all sources in APA style as outlined in the Ashford Writing Center (for more information about how to create an APA reference list, take a look at the APA References List webpage.
• Must include a separate references page that is formatted according to APA style as outlined in the Ashford Writing Center.

Carefully review the Grading Rubric for the criteria that will be used to evaluate your assignment.

 

PHI103 Final Paper Options Here

is the list of options from which to choose your paper topic. The idea is to use the same topic for all three papers in this course, developing and improving your argument as you go. If you want to switch topics between papers or choose a topic not on this list, consult with your instructor first. It is also possible for your instructor to add other options to this list, so check the instructorâ€s announcements for any modifications. 

 â€¢ Is it ever permissible to torture a person?  • Should athletes be allowed to use performance-enhancing drugs (such as steroids or EPO) or techniques (such as blood doping or oxygen tanks)?   • Should universal healthcare be available to all regardless of their ability to pay in the same way that other services (such as education) are available to all for free?   • Does one have an obligation to report wrongdoing by oneâ€s employer, even when doing so will result in the loss of oneâ€s job?  Are whistle blower laws effective and necessary? • Does social media enhance or hinder interpersonal relationships?   • Is it important to teach the arts and humanities

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