Can you suggest a possible difference between the two peptides that might account for the observed m/z difference?

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You have isolated the proteins from two other adjacent spots after 2D electrophoresis and digested them with trypsin. Following MALDI-TOF mass spectrometry, the 2 two proteins were found to be identical except for one peak. For this peak, the mass-to-charge ratio (m/z) values differed by 80 g/mol (or daltons), a value that does not correspond to any discrete difference in amino acid sequence. (For example, glutamic acid i instead of valine at one position would give an m/2 ーーーーーーーーーーーーーーー difference of around 30.) Can you suggest a possible difference between the two peptides that might account for the observed m/z difference? m/z (mass to chare ratio)

You have isolated the proteins from two other adjacent spots after 2D electrophoresis and digested them with trypsin. Following MALDI-TOF mass spectrometry, the 2 two proteins were found to be identical except for one peak. For this peak, the mass-to-charge ratio (m/z) values differed by 80 g/mol (or daltons), a value that does not correspond to any discrete difference in amino acid sequence. (For example, glutamic acid i instead of valine at one position would give an m/2 ーーーーーーーーーーーーーーー difference of around 30.) Can you suggest a possible difference between the two peptides that might account for the observed m/z difference? m/z (mass to chare ratio)

 

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