microbiology 17 – Quality Nursing Writers

1. Read the Lab Protocol found on pages 173-182 of Microbiology Laboratory Theory and Application by Leboffe and Pierce. It’s important that you read the lab and data sheets fully to understand the scope of the lab activity.
Visit Staining Microscopic Specimens for comparisons about different staining techniques, images, and practice questions to check your understanding.
You may want to Google the effects of over- and under- staining bacterial smears.
Check out this interactive scale showing the size of cells Cell Size and Scale
Check out this Wikipedia entry for the largest bacterium (that we’ve discovered so far!) – Thiomargarita namibiensis
You MUST show your work for the surface area and volume calculations, but you can check your calculations using these links for volume and surface area.
Submitting your work:
Lab 3-4 Simple Stains
2. Read the Lab Protocol found on pages 187-194 of Microbiology Laboratory Theory and Application by Leboffe and Pierce. It’s important that you read the lab and data sheets fully to understand the scope of the lab activity.
Watch this demonstration on How to Perform a Gram Stain, and note the instructions on oil immersion microscopy.
Watch this link on Gram Staining from Khan Academy
Here is a direct link to Google Images search
Submitting your work:
Lab 3-6 Gram Stain

https://bookshelf.vitalsource.com/#/books/9781617315602/cfi/1!/4/4@0.00:0.00 (Microbiology Laboratory Theory and Application lab manual (where to find the pages))

 
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