Need help with my writing homework on DWrite a 1250 word paper answering; Membrane lipids possess a critical common structural theme: membrane lipids are amphipathic molecules (amphiphilic molecules), most of which form bilayers. Membrane lipid contains both a hydrophilic (water-loving) and a hydrophobic moiety. In essence, hydrophilic molecules dissolve readily in water because they contain charged groups or uncharged polar groups that can form either favorable electrostatic interactions or hydrogen bonds with water molecules. Hydrophobic molecules, by contrast, are insoluble in water because all, or almost all, of their atoms, are uncharged and nonpolar and therefore cannot form energetically favorable interactions with water molecules.

Question 2

(a) What type of secondary structure is adopted by the polypeptide segments Met 1–Ala 11 and Thr 50–Ala 59? (2 marks)

(b) What type of secondary structure is adopted by the polypeptide segment Ile 93–Arg 102? (1 mark)

(c) How many chiral centers are present in the C-terminal amino acid residue? (1 mark)

(d) At the normal cellular pH, what will be the charge of amino acid residue 62? (1 mark)

(e) In this model of isolated, purified protein none of the cysteine residues has formed disulphide bonds. Assuming that Ras adopts the same conformation within the cell, give two reasons why internal disulphide bonds are unlikely to form in this protein. (2 marks)

(f) The guanine base of the GDP forms hydrogen bonds with Asp 119 in Ras.

(i) How many hydrogen bonds are shown in the model between GDP and Asp 119? (1 mark)

(ii) What functional group of the amino acid forms the bonds? (1 mark)

(iii)One hydrogen bond is formed between the nitrogen atom N1 in the guanine base and the oxygen atom OD1 in the Asp residue (N—H—O). What is the distance between the nitrogen and oxygen atoms? (1 mark)

(g) Is this form of Ras active or inactive? Explain your reasoning. (2 marks)

(h) If site-directed mutagenesis was carried out on H-Ras to change Lys 16 to Tyr 16, what effect do you think this would have on the function of the protein? Explain your reasoning. (2 marks)

(i) Outline what effect interaction of Ras with an appropriate GEF (guanine nucleotide exchange factor) would have on the structure and function of Ras. (2 marks) (You need to upload the relevant data).

Complete 2 pages APA formatted article: Reflection on the Research Paper. The research was not easy. I found it difficult because as I was researching the causes of Wal-Mart’s failure and relating it to theory, I discovered that there were more causes and theoretical explanation than could possibly fit into the given word count. It was then that I realized that in this assignment we were probably being tested on our ability to isolate the more important causes for failure, as in the root causes, as opposed to listing all those identified.

The difficulty encountered and the realization I made as a result were important learning experiences. This is because, as I was reading through the articles, I made a list of all the cited causes. To be honest, my main concern was the identification of all causes and writing as comprehensive a research as possible. As this strategy turned out to be incorrect, I had to reread the articles with a different purpose in mind – the identification of the root causes. As none of the articles accessed or handed out as part of our class readings outlined the root causes for the failure of retail chain internationalization projects, I had to reflect upon the various causes, establish correlations between them, and relate them back to theory. Not only did doing so give me a deeper understanding of the problems confronting the internationalization of retail chains but, it gave me a better grasp of theory. Accordingly, the very nature of the assignment, and the supposed imbalance between word limit and the vast array of causes for failure, were learning experiences in themselves.

As my initial approach had been incorrect, forcing me to reread each article more than once, I gained a greater familiarity with the different writers in the field, to the extent that I was able to compare the worth of each. While not claiming that any of the writers or theorists whose material we studied lacked substance or value, I personally found the most informative, hence more valuable, to be Brown and Palmer. Both explained the relevant theoretical assertions in both straightforward and comprehensive terms, allowing readers, not only to understand the theories in question but, more importantly to apply them. It was primarily due to Palmer and Brown’s articles that I was able to understand, and subsequently apply, the concepts of strategic fit, divestment and the Big Middle to Wal-Mart’s failure in Germany. Indeed, Brown and Palmer, but especially the latter, gave me an understanding of the assignment which I lacked when I started out.

As a final thought, I believe that I successfully fulfilled the assignment’s objectives. As I interpreted it, the objective was not the identification of the causes of failure but the more important root causes. Similarly, the aim of the assignment was not simply the understanding of the relevant theoretical propositions but the development of the ability to apply theory to real cases. As a I fulfilled both these objectives, in closing, I can stress that the assignment proved a valuable learning experience.