immunological basis of disease

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I need some assistance with these assignment. immunological basis of disease Thank you in advance for the help! a. Comparison of bronchial tissues from asthmatic and non-asthmatic patients by bronchial examination and biopsy. The immunohistochemistry of the proximal airway of bronchial biopsies was conducted by staining them with monoclonal antibodies against smooth muscle actin, and tryptase for mast cells, followed by image analysis.

b. Measuring the effect of the coculturing of mast cells and ASM on the production of smooth muscle actin and TGF-β. Pure cultures of human lung mast cells and airway smooth muscle were produced. ASM cells were labeled with mABs for smooth muscle actin (SMA). ASM cells were cultured with human mast cells, and mast cells that have been sensitized with protease inhibitor and lactoferrin (to inhibit the action of tryptase, a protease produced by mast cells). Under these coculture conditions, the production of smooth muscle actin protein and gene expression were determined periodically for one week using immunofluorescence, flow cytometry, and quantitative real-time PCR. Cell-free supernatants of the cocultures were analyzed for the production of TGF-β1 and TGF-β2 using ELISA. TGF-β ( transforming growth factor-beta) is a protein that controls cell proliferation and differentiation

c. The effect of the addition of recombinant TGF-β on ASM proliferation and contractile activity of ASM pure cultures was also measured by examining SMA protein expression by flow cytometry, and ASM contractile activity by collagen gel analysis.

3. Choose ONE immune-based method used in the paper and, with reference to the paper and the literature, describe this method including its underlying principles. (Use diagrams where appropriate). (No more than 500 words excluding diagram(s)) (30%).

An immune-based method used in the paper was ELISA or enzyme-linked immunosorbent assay. In the paper being reviewed, ELISA was used to quantify the expression of TGF-β1 and TGF-β2 in supernates of ASM and mast cell cultures.

Complete 8 pages APA formatted article: The Relevance of Electronic Data Interchange For E-Business Today.

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Complete 8 pages APA formatted article: The Relevance of Electronic Data Interchange For E-Business Today. When organizations implement EDI in payment of employees, especially in companies and banks, this institution uses the term FEDI (Financial Electronic Data Interchange) (Schneider 2013, p. 138). For business managers, with the current use of extranets, intranets, digital currency, and electronic catalogues, they should acknowledge EDI’s relevance for e-business today. However, implementation of EDI in a business organization not only brings positive impacts, but it also has some negative impacts, which can cause negative effects to users who are using recent versions of documents instead of a business partner (McGrath 2010, p. 76). The aim of this report is to enlighten business owners the relevance of EDI in e-business today.

EDI is the electronic communications or exchange of business transactions between business organizations. It entails computer-to-computer communications directly to order systems and vendor databases. In the year 1996, the NIST defined EDI as a computer-to-computer transaction of severely formatted information representing documents rather than financial instruments. It means exchange of a series of messages between two or more parties, whereby one serves as a sender and the one a receiver (Zainol 2009, p. 256). The originator transmits the formatted data that represent a document to the receiver through physical transportation on electronic media storage such as CDs or USB Flash disks or via telecommunication (Zekos 2008, p. 67).

Despite the relative advantages of EDI, most business organizations still have challenges in implementing EDI in their operations. However, the entire process of implementing EDI is complex and this is the reason why there is still a large group of organizations that uses the traditional mode of transmitting transactions (Zainol 2009, p. 259).&nbsp.

In addition, the process of implementing EDI and training employees to achieve the necessary skills to use these information systems is extremely costly.&nbsp.

Information Systems

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After completing the reading this week answer the following questions: Chapter 3: Note the basic concepts in data classification.Discuss the general framework for classification.What is a decision tree and decision tree modifier? Note the importance.What is a hyper-parameter?Note the pitfalls of model selection and evaluation.Read: Chapter 3 in textbook: Classification: Basic Concepts and Techniques Sample […]

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Applying an ethical decision making model

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Most often we encounter ethical dilemmas related to end-of-life issues. The purpose of this forum is to practice applying the 6.0 Model of Ethical Reasoning PHI 1600 Applied Ethics Dr. Dave Monroe (linked here). This decision framework was introduced in Week 1. You will apply it to an ethical dilemma you encountered, or have personal […]

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