Compose a 3500 words assignment on biotechnological applications of cultivated and uncultivated marine microorganism.

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Compose a 3500 words assignment on biotechnological applications of cultivated and uncultivated marine microorganism. Needs to be plagiarism free! Study of the application of a particular marine microbe is succeeded by a number of identification and characterisation studies. Marine microbes are studied and identified based on identification, enumeration, activity, colony structure. The study of microorganisms is based on the identification techniques. Identification is essential for the classification of microorganisms. The various identifications methods for the cultivated microorganisms include morphological identification, differential staining, differential media, serological methods and flow cytometry. Identification of uncultivated microorganisms is based on the protein analysis and the comparison of the nucleotide sequences. Enumeration is a methodology used to estimate the number of different microorganism in a given sample. It is either achieved through the direct enumeration technique and other modern filter techniques. Direct enumeration method utilises microscope to count the number of microbes in a given sample. There are many other methods for enumeration, including minimum dilution, MPN method, most probable number methods, and plate count methods using agar media or silicic acid gels. The selection of any of the method is based on the species and nature of the microorganism being studied. For instance, the MPN method is used for the enumeration of the hydrocarbon-degrading bacteria in the marine environment. In the marine environment, various microorganisms are related to diverse activities. For instance, biosurfactant producing marine bacteria can be studied through haemolytic assay (HA), modified drop collapse (MDC), tilted glass slide, oil spread method (OSM), blue agar plate(BAP), emulsification index(EI) and emulsification assays. Munn showed that the study of the community structure and the allocation of function to different groups of microorganisms could be achieved through microelectrodes and biosensor methods.

 

creating a thesis and an outline on Consider Ethics: Theory, Readings, and Contemporary Issues. Prepare this assignment according to the guidelines found in the APA Style Guide. An abstract is required.

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I need help creating a thesis and an outline on Consider Ethics: Theory, Readings, and Contemporary Issues. Prepare this assignment according to the guidelines found in the APA Style Guide. An abstract is required. Using the case scenario given below, we are going to tackle the impact and the boundaries of social workers in the ethical decision-making of another individual. We are given a case of Mr. M, a fifty-six-year-old man who lives in a residential home, who was placed in the care of the institution by his family. Unlike most elderly, Mr. M is special in the sense that he had been born with the congenital disorder called Trisomy 21, which is otherwise known as Down Syndrome. This is a chromosomal dysgenesis syndrome consisting of a variable constellation of abnormalities caused by triplication or translocation of chromosome 21. The abnormalities include mental retardation, retarded growth, flat hypoplastic face with short nose, prominent epicanthic skin folds, small, low-set ears with prominent antihelix, fissured and thickened tongue, laxness of joint ligaments, pelvic dysplasia, broad hands and feet, stubby fingers, and transverse palmar crease. Lenticular opacities and heart disease are common. The incidence of leukemia is increased and Alzheimer disease is almost inevitable by age 40. In this respect, Mr. M is often not able to take care of himself, and has trouble remembering many things, and has had to take maintenance medications for years. When at his best behavior, he will take the medicine without any problems. When he is not in a very good mood, he would often refuse the medications. When this occurs, the caregivers would decide to hide the medications in Mr. M’s food and he would unknowingly be able to take his medications for that day. We will now try to see whether these actions present ethical dilemmas. First, we would have to understand that in his current condition, Mr. M is obviously challenged in many respects, but the most prominent of these is his mental retardation.

 

submit a 750 words paper on the topic Assignment 5-2. Mistakes are relatively low on cost and therefore, less serious.

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Hi, need to submit a 750 words paper on the topic Assignment 5-2. Mistakes are relatively low on cost and therefore, less serious. Creative or lateral thinking is an alternative perspective on the situation. and thus the problem and solution states can be interpreted differently from how most people see it. This leads to an alternate style of responding to the problem. A novel solution can often seem more interesting, and people are likely to become involved in the process with more enthusiasm. thereby leading to a greater chance for success. Which style of information processing should be used in a given situation or is more likely to succeed should be chosen carefully by the person seeking to solve a problem they face in the workplace. In the present scenario, vertical processing and problem solving techniques were used to resolve a reoccurring problem in the office. It was noted that attendance of employees dropped drastically when there was a particular type of sporting event involving major regions or the country. During the previous sport season, there was a distinct drop in the productivity of the set-up. When another season was due. this history became a major concern for the manager. It was decided that a few key employees should be involved in finding a solution to the problem of reduced productivity. Upon discussion, it was found that the younger employees are more likely to want to take a day of to watch sports. After this conversation, it was decided that certain key playing days should be announced half – day working on the promise that other day’s would not be affected. Accordingly, a notice was circulated amongst the staff so that they feel motivated enough to come for the rest of the days without taking an unannounced (impromptu) holiday. On understanding the process of using lateral thinking it was believed that there could be other alternatives to the solution to this problem. First of all the assumptions made when evaluating the problem were tested. The manager found that the assumption that all productivity dropped on these target days was untrue. Only certain members of the staff were implicated in this situation. The other assumption that distraction was due to the game was also challenged, since the rest of the staff was not interested in abstaining from coming to work, or working at the same level a s any other day. If a reasonable number of staff were not planning to watch sports, they could easily continue work. But it is rather unfair to give free time only to a few employees because they want to watch a sport. Thus, a more right question needs to be asked by the manager as to what options are there so that it is a win – win situation for all. A new and alternate approach to the situation is to tune the cafeteria television to the Sports channel, and allow the employees watch the game for its said time-period. This option, though unconventional, at least keeps the employees at work, and only those who wish to see them do view the sports. Also, in the event that another employee needs them to be around when getting a task done. this work will not stagnate due to the unavailability of the employee in question. Although any screen which can be connected to the office can be used to relay the match to the employees. it makes logical sense to select the one in the cafeteria, as the noise and cheering will not trouble other employees who may not be interested in the game.

Microbe on Escherichia coli. The work is to be 2 pages with three to five sources, with in-text citations and a reference page.

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I will pay for the following article Microbe on Escherichia coli. The work is to be 2 pages with three to five sources, with in-text citations and a reference page. Part I: Organism Information Organism Escherichia coli Organism Type: Bacteria Organism Structure: E. coli is a gram-negative bacterium with rod shaped cells 2.0 (μm) long with a diameter of 0.5 (μm). The bacterium grows best at 37°C but some strains have successfully multiplied in controlled laboratory settings at even 49°C. It is a facultative anaerobe reproducing using mixed-acid fermentation requiring low hydrogen levels to ferment simple sugars into formic acids. E. coli is also found with other hydrogen consuming organisms like sulphate-reducing bacteria (Hayhurst, 2004).

Organism Characteristics: The bacterium is usually found in the lower intestinal tract of warm-blooded animals and humans. Most of the strains found in the gut are actually beneficial to the host by inhibiting the growth of other harmful bacteria and synthesizing vitamins, like K2. However, some serotypes can cause severe food poisoning in humans. One common strain, O157:H7, releases strong toxins, specifically enterotoxins, that damage and infect the host’s intestinal lining (Rasko, 2011). At times, a small colony of only 10-100 cells in enough to cause infections in children. The incubation period of these types of infection causing strains can range anywhere from a few hours to a week. The bacteria penetrate and thrive in the intestinal lining, eating away at the mucosa (Hayhurst, 2004).

Part II: Disease Information

Diseases caused: Food poisoning

Mode of Transmission: Usually through fecal-oral transmission and coming in contact with contaminated foods and drinks

Portal of Entry into body: Main route through oral ingestion

Area of body organism attacks: Mucous lining of the intestines

Symptoms of Disease: Symptoms usually manifest themselves in adults after 3-4 days of being infected consisting mostly of mild diarrhea, abdominal cramping and nausea. In severe cases the diarrhea can become bloody and the infection can cause kidney problems leading to pale skin, fever, chills, and bruising. Most healthy adults get better within a weak while for infants and young children it can take longer. In rare and extreme cases the virulent strain can also cause pneumonia, hemolytic uremic syndrome (kidney failure), and dehydration through diarrhea, eventually leading to death in young children and older adults (Hayhurst, 2004).

Part III: Treatment and Prevention

Diagnosis: Usually the doctor carries out a physical examination and a medical history involving a series of questions about many of the symptoms. The questions will also try to determine travelling history, recently eaten foods, contact with contaminated foods and unpasteurized dairy products, and antibiotic use. The physical examination consists of checking the patient’s temperature, blood pressure, skin color, stomach tenderness and a rectal exam. If E. coli infection is suspected, the doctor will request a stool culture examination to determine the presence of the infectious strain and its toxins (Rasko, 2011).

Treatment: There aren’t any medications and vaccines yet to protect against E. coli infections. One of the best methods to relieve symptoms is to get plenty of rest and fluids in order to decrease dehydration due to diarrhea. If bloody diarrhea occurs, diarrhea medicine and antibiotics should be avoided as these slow the digestive system, allowing even more of the poison produced by the bacteria to be absorbed. In extreme cases where kidney failure or blood problems occur, blood transfusion and dialysis will be needed (Hayhurst, 2004).

Prevention: The primary way to prevent infections is to limit the exposure to contaminated foods and water, unpasteurized dairy products, and avoiding cross-contamination. Meats should be cooked thoroughly at high temperatures and raw fruits and vegetables should be washed before use. Hands should be washed before and after preparing food, using the washroom and coming into contact with animals. When travelling, it is best to avoid ice and tap water (Hayhurst, 2004).

Part IV: Historical information

1885: Theodor Escherich, a German pediatrician, discovered E. coli and named it Bacterium coli commune because it was found in the colon of healthy adults (Hayhurst, 2004).

1917: Nissle, a nonpathogenic strain of E. coli, started being used in medicine to treat many gastroenterological diseases like, inflammatory bowel disease (Hayhurst, 2004).

1997: The DNA sequence of E. coli was first completed and published. The circular DNA was found to have 4.6 billion base pairs and a very high coding density (Hayhurst, 2004).

2011: An outbreak of the strain O104:H4 began in Germany leading many people to be infected with hemolytic-uremic syndrome. Gradually 11 other countries were affected including some parts of North America. Later that year, the Federal Institute for Risk Assessment in Germany announced that fenugreek seeds from Egypt were the likely cause of the outbreak (Rasko, 2011).

Part V: Works Cited

Hayhurst, Chris. (2004). Epidemics, Deadly Diseases Throughout History: E. coli. New York, NY: The Rosen Publishing Group, Inc.

Rasko, D. A., et al. (2011). Origins of the E. coli Strain Causing an Outbreak of Hemolytic–Uremic Syndrome in Germany. The New England Journal of Medicine, 365, 709-717. Retrieved from http://www.nejm.org/doi/full/10.