Create a 5 pages page paper that discusses antibiotics and agar diffusion. The agar plate diffusion methods are assays used to observe and quantify the antimicrobial property of tested substances. Briefly, the newly inoculated agar is placed with a small piece of paper soaked with the agent. After incubation, antimicrobial substances should produce a sterile ring surrounding the soaked paper. The diameter of the ring is an indication of the strength of the substance’s antimicrobial property. The aim of this experiment was to determine the effects of agar depth, inoculum size, presence of salts, and method of applying antibiotics on inhibition zones.

In determining the effect of agar depth on the result of agar plate diffusion, four plates containing 10 cm3, 20 cm3, 20 cm3, and 30 cm3 agar were first swabbed with Escherichia coli 10418 (1:20 dilution). All plates were oriented horizontally except for the third one, which was slanted. Meanwhile, 2 plates each containing 20 cm3 DSTA were inoculated with 100 µl of either 1:20 E.coli dilution or undiluted broth culture. 4 discs each containing 20 µl 0.5 mg/ml ampicillin were placed in each of the plates. The plates were incubated overnight at 37°C.

For determining the effects of potassium or calcium ions on zone sizes, 0.1 or 1.0 ml of 2M KCl or CaCl2 were added onto their corresponding plates each containing 20 ml DSTA, which would be inoculated with 0.1 ml Pseudomonas aeruginosa (1: 50). 4 discs each with 20 µl of either 20 mg/ml ampicillin (A) or 1 mg/ml streptomycin (S) were then placed on the agar plates. The plates were incubated overnight at 4°C

In observing the difference between two methods of antibiotic application, 8 discs were soaked with 0.5 mg/ml ampicillin, while another 8 were each added with 20 µl of the antibiotic solution. The discs were distributed equally to four DSTA plates inoculated with Staphylococcus aureus.

Hi, need to submit a 250 words paper on the topic The Role of Supplier Relationships in Supply Chain Management. Supplier relationships are important to supply chain management. The relationship between business and supplier must be cultivated at all costs. If a manufacturer and supplier have a good relationship solution can be found within the relationship. This allows for personal contact between manufacturer and supplier in order to solve any conflicts that might arise (Fynes et al., 2005). A manufacturer depends on suppliers. If a supplier does not deliver than trust is lost. Once trust is lost, the manufacturer and supplier relationship suffer.

The Porter Five Force state five forces impact a business. The five forces are bargaining power of customers, the threat of new entrants, bargain power of suppliers, the threat of substitute products, and competitive rivalry within the industry. A supplier’s bargain power ranks with customer power, the threat of new entrants, substitute products, and rivalry. Supplier bargaining power is important. That is why the supplier and manufacturer relationship is so important.

Input prices deviate from those that would prevail in a perfectly competitive input market in which input suppliers act as price takers due to the lack of competition. When the market has three major suppliers, the individual suppliers cannot negotiate prices. If the individual suppliers get contracts, the main goal is keeping the business. The larger suppliers can give bigger discounts due to the volume of business done. The individual suppliers cannot afford to not make a profit, whereas a bigger supplier can absorb profit loss better. Thus bigger suppliers can provide lower prices than individual suppliers. In a competitive input market, the product can be offered at a level rate. For example, if product X costs a specific amount to make. Suppliers would be able to offer product X for about the same amount.

Suppliers can make or break a business. Suppliers only have a concern for the individual business in regard to what will happen to their supply business. Suppliers are only as good as the supply of product given for the least amount of money. If a business fails, the supplier can find someone else to supply. Businesses rely on good suppliers.