Out-of-Class Activity 2: Invisible Knapsack

For this activity, you will read Peggy McIntosh’s article “White Privilege: Unpacking the Invisible Knapsack” (1990) and write at least a 450-word reaction paper. You must upload your assignment to Canvas on the day that it is due.

If you would like feedback on your assignment, in addition to uploading your assignment on Canvas, you must submit a hard copy of it at the beginning of class on the day that it is due. (Inversely, if you do not wish to receive feedback, and you only want to receive your grade, you should only upload your assignment to Canvas.)

The article is on Canvas under Files, Assignments, Out-of-Class Activities, OCA 2, “McIntosh – White Privilege Unpacking the Invisible Knapsack.”

Questions to Address for this Activity:

1. Reflect on the article as a whole.
   1. What are your initial reactions? How did reading it make you feel?
2. Think about the McIntosh’s goal in writing this article.
   1. What is the author trying to do? Do you feel that she did a good job of it?
   2. Is there anything you would say particularly different than McIntosh?
      1. Keep in mind when answering this the year in which the author wrote the article – context is always important, even when we are analyzing and/or critiquing work.
   3. Next, I want you to reflect on “the items in the knapsack” listed in the article.
      1. Have you thought about these situations before? Never? Sometimes? Constantly? Depending on the frequency, answer one of the following:
         
         1. If you have never really had to think about these situations, how do you think it feels to be aware of these situations? How would that shape your daily interactions with others and how you see and think about yourself?
         2. If you have to think about these situations sometimes or constantly, how does that feel? How do you think your daily life and understandings of yourself would be different if you didn’t have to worry about these situations?
   4. Lastly, I want you to add one situation, statement, idea, etc. to McIntosh’s list that she does not have on hers. Discuss briefly why your contribution should be on the list.
      1. NOTE: I will deduct points if your contribution is the same as one of hers or if it is too closely related to one of hers.

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Identify control and experimental samples, as well as independent and dependent variables.
Addresses course outcomes 1-4:

recognize and explain how the scientific method is used to solve problems
make observations and discriminate between scientific and pseudoscientific explanations
weigh evidence and make decisions based on strengths and limitations of scientific knowledge and the scientific method
use knowledge of biological principles, the scientific method, and appropriate technologies to ask relevant questions, develop hypotheses, design and conduct experiments, interpret results, and draw conclusions
This is the culminating lab assessment in BIOL 103. It is designed to assess your ability to apply the principles of the scientific method.

The Effect of low pH on Enzyme Activity

Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project, it may be useful for you to first review the Scientific Method Tutorial, the OLI module about pH and enzymes, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there are several factors that impact enzymatic activity: pH, temperature, and amount of reagent. It is OK to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment), or you can search on-line to find a different enzyme/substrate/method for measuring enzyme activity for your project (include all references).

As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design, you must clearly explain what you are doing. That means that you will need to identify the enzyme, the substrate, the acidic fluid used as treatment, the control treatment and the method of measuring enzyme activity, as well as explain your experimental protocol. You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment as well as knowledge of enzymes and pH from the OLI modules, lab manual and potentially additional information sources.

Hint: Keep in mind that the acid will change the environmental conditions of the experiment (for example, a low pH value could change the shape of the active site on the enzyme protein), without directly participating in the reaction.

Lab Materials

You may need all or some of the following, depending on your experimental design:

Materials from your lab kit:

pH paper
hydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the bottle that came with the lab kit)
plastic beakers or cups
vinegar
yeast (can be purchased at grocery store if more is needed)
balloons
plastic bottle
marker for labeling of beakers
Cell phone/digital camera – pictures required
You may choose to use additional materials (different acidic fluids and/or different organisms and/or different substrate if you chose an enzyme other than catalase).

Outline:

. .

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